A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing
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چکیده
منابع مشابه
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Multiplex PCR/Reverse Line Blot Hybridization assay allows the detection of up to 43 molecular targets in 43 samples using one multiplex PCR reaction followed by probe hybridization on a nylon membrane, which is re-usable. Probes are 5' amine modified to allow fixation to the membrane. Primers are 5' biotin modified which allows detection of hybridized PCR products using streptavidin-peroxidase...
متن کاملEnhanced multiplex PCR assay for typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus.
We previously published a simple multiplex PCR assay for quick-screening of Staphylococcal Cassette Chromosome mec (SCCmec) types I-V, which has been extensively used worldwide. An updated assay is described here and the changes serve to make this rapid assay more accurate and reliable in facilitating identification of most common and major SCCmec types.
متن کاملNovel PCR-restriction fragment length polymorphism analysis for rapid typing of staphylococcal cassette chromosome mec elements.
We developed a novel PCR-restriction fragment length polymorphism test for the ccrB gene by using HinfI and BsmI for rapid typing of staphylococcal cassette chromosome mec (SCCmec). When tested with reference strains and methicillin-resistant Staphylococcus aureus isolates, the method proved to be valid and useful for rapid identification of four SCCmec types, especially type IV.
متن کاملStaphylococcal cassette chromosome mec (Sccmec) classification and typing methods: an overview.
Meticillin-resistant Staphylococcus aureus (MRSA) is one of the main causes of hospital-acquired infections, but since late 1990s also the community-acquired. For better understanding of the S.aureus epidemiology there is an urgent need for creation of new typing method for SCCmec element. The molecular typing of MRSA for epidemiological purposes is investigated by pulsed-field gel electrophore...
متن کاملMolecular Investigation of Staphylococcal Cassette Chromosome mec (SCCmec) Elements Isolated from Intensive Care Unit
Background and Aims: Based on the results, Staphylococcus aureus is one of the serious infectious agents found in community and hospitals with remarkable potential for high morbidity and mortality around the globe. The present study was carried out for molecular investigation of methicillin-resistant Staphylococcus aureus strains and Staphylococcal Chromosomal Cassette mec (SCCmec) phenotypes i...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2009
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.007955-0